APPLICATION OF HMB－45 MONOCLONAL ANTIBODY AND S100 PROTEIN IN THE IMMUNOHISTOCHEMICAL DIAGNOSIS OF MELANOMA

ＡＰＰＬＩＣＡＴＩＯＮＯＦＨＭＢ－４５ＭＯＮＯＣＬＯＮＡＬＡＮＴＩＢＯＤＹＡＮＤＳ１００ＰＲＯＴＥＩＮＩＮＴＨＥＩＭＭＵＮＯＨＩＳＴＯＣＨＥＭＩＣＡＬＤＩＡＧＮＯＳＩＳＯＦＭＥＬＡＮＯＭＡＬｉｕＤａｎｙａ（刘丹亚）；ＳｕＢａｏｓｈａｎ（苏宝山）；Ｘｕ...     

100%低地板现代有轨电车轻量化研究

100%低地板现代有轨电车具有节能、环保、投资小、载客量适中、乘坐舒适性高、后期维护费用低等特点,同时面临着严峻的轻量化及减重要求。对车体结构轻量化以及车体部件和内饰部件轻量化材料的应用开展研究,介绍了相关设计结构及应用方案。     

幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

WWOX蛋白在非小细胞肺癌中的表达及其临床意义

目的 探讨WWOX(WW domain-containing oxidoreductase)基因在非小细胞肺癌组织中的表达及其临床意义.方法 用免疫组化方法检测50例鳞癌、31例腺癌及20例癌旁正常组织中WWOX基因蛋白的表达,并分析其与各临床病理指标之间的相关性.结果 WWOX基因在72.8%的非小细胞肺癌中失表达或表达减少,而在相邻正常肺组织中有80.0%正常表达.WWOX基因的表达与组织类型、细胞分化程度密切相关(P<0.05),在鳞癌和低分化癌中WWOX基因失表达或表达减少.结论 WWOX蛋白在非小细胞肺癌组织中呈低表达,该蛋白表达的缺失可能在不同类型非小细胞肺癌的形成中发挥了不同的作用.     

AMP激活的蛋白激酶对大鼠弥漫性轴索损伤后神经元自噬的调节

目的研究AMP激活的蛋白激酶(AMPK)信号通路在弥漫性轴索损伤(DAI)大鼠脑组织中的表达,探讨AMPK对DAI后神经自噬的调节机制。方法大鼠头颅瞬间旋转损伤法建立DAI模型,免疫蛋白印迹法检测造模后1、3、7d大脑皮层和海马AMPK、磷酸化AMPK(p-AMPK)、Beclin1、LC3Ⅰ/Ⅱ、p62蛋白的动态表达,并与正常组和假手术组比较。侧脑室给予AMPK抑制剂Compound C及激活剂AICAR,检测药物干预对大鼠神经自噬相关蛋白表达的影响,并与DAI组和vehicle组进行比较。结果与正常组和假手术组相比,DAI 1d后脑内AMPK信号通路激活,3d后脑内自噬水平上调;Compound C可以减少AMPK磷酸化激活同时下调脑内的自噬水平,AICAR可以增高AMPK磷酸化水平上调神经自噬改善神经功能损伤。结论大鼠DAI后AMPK的激活可以上调神经自噬的水平,对DAI后的神经保护有重要意义。     

THE QUANTITATIVE MEASUREMENT OF BCL-2, P53 PROTEIN AND PCNA EXPRESSION IN BREAST CARCINOMA AND THEIR CORRELATION WITH PROGNOSIS

Recently,alargenumberofresearchesindicatedthatoccurenceanddevelopmentofbreastcarcinomaswerecorrelatedwithhighexpressionofblockingapoptosisoncogene,mutationofantioncogeneandexpressionofcontrollingcellproliferativeoncogene.Therewerefewreportsondocumentsofquantitativestudyofexpressionaboutblockingapoptosisoncogene--hcf--2(Bcellleukaemia/lymphomagene),productofP53proteinandproliferatingcellnuclearantigen(PCNA)inbreastcarcinoma.Ourpurposewastoinvestigatequantitativeindexinrelationtoclinicalpathol…     

100 m定尺钢轨基地焊接500 m长钢轨施工技术

京津城际铁路设计采用100 m定尺钢轨基地焊接成500 m长钢轨,再用500 m长钢轨一次性铺设、焊接、锁定成跨区间无缝线路,引进Gaas80/580闪光接触焊轨机及配套的除锈、四向调直、精磨等成套设备,对100 m定尺钢轨焊接500 m长钢轨的焊轨生产线布局、焊轨流程及工序、质量控制要点等进行介绍.     

Initial results from long-term measurements of atmospheric humidity and related parameters in the marine boundary layer at two locations in the Gulf of Mexico

Measurements of boundary layer moisture have been acquired from Rotronic MP-100 sensors deployed on two NDBC buoys in the northern Gulf of Mexico from June through November 1993. For one sensor, which was retrieved approximately 8 months after deployment, the post- and precalibrations agreed closely and fell well within WMO specifications for accuracy. The second sensor operated continuously from June 1993 to February 1997 (3.5 years). Buoy observations of relative humidity and supporting data were used to calculate specific humidity and the surface fluxes of latent and sensible heat. Specific humidities from the buoys were compared with observations of moisture obtained from nearby ship reports, and the correlations were generally high (0.7–0.9). Surface gravity wave spectra were also acquired. The time series of specific humidity and the other buoy parameters revealed three primary scales of variability, small (h), synoptic (days), and seasonal (months). The synoptic variability was clearly dominant and occurred primarily during September, October, and November. Most of the synoptic variability was due to frontal systems that dropped down into the Gulf of Mexico from the continental US followed by air masses which were cold and dry. Cross-correlation analyses of the buoy data indicated that: (1) the moisture field was highly coherent over distances of 800 km or more in the northern Gulf of Mexico; and (2) both specific humidity and air temperature served as tracers of the motion associated with propagating atmospheric disturbances. These correlation analyses also revealed that the prevailing weather systems generally entered the buoy domain from the South prior to September, but primarily from the North thereafter. Spectra of the various buoy parameters indicated strong diurnal and semidiurnal variability for barometric pressure and sea surface temperature (SST) and lesser variability for air temperature, wind speed and significant wave height. The surface fluxes of latent and sensible heat were dominated by the synoptic events which took place from September through November with the transfer of latent heat being primarily from the ocean to the atmosphere. Finally, an analysis of the surface wave observations from each buoy, which included calculations of wave age and estimates of surface roughness, indicate that major heat and moisture flux events coincide with periods of active wave growth, although the data were insufficient to identify any causal relationships.     

基于CPLD的0.01s计时器设计

本设计提出了一种基于CPLD的0.01S计时器的设计方案。应用高速集成电路以硬件描述语言VHDL语言描述,采用Altera公司的可编程逻辑器件(CPLD)EPM7064SL84实现的现代智能计时仪表,为广大用户提供了方便。系统具有可靠性高,便于功能扩展,成本低,易普及等优点。